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http://hdl.handle.net/123456789/1513| Title: | Screening Mutants by Single Fly Genomic PCR |
| Authors: | Nagarajan, U Georgiou, M |
| Issue Date: | Jul-2022 |
| Abstract: | Imprecise P-element excision or FRT-mediated recombination is routinely performed to mutagenize a gene of interest. It is, however, tedious to maintain all independent and individual excised mutant fly lines before the presence of a mutation is confirmed. Here, we provide a method to detect and confirm the presence of a mutation, as and when mutant flies are generated. By allowing for the maintenance and expansion of only the confirmed mutant lines, this protocol will help to save time, money, and space. |
| URI: | http://hdl.handle.net/123456789/1513 |
| Appears in Collections: | School of Interdisciplinary & Applied Sciences |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 2022-Nagarajan-Georgiou2022_Protocol_ScreeningMutantsMutantsBySingl.pdf | 485.46 kB | Adobe PDF | View/Open |
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