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http://hdl.handle.net/123456789/1513Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Nagarajan, U | - |
| dc.contributor.author | Georgiou, M | - |
| dc.date.accessioned | 2024-05-15T06:33:20Z | - |
| dc.date.available | 2024-05-15T06:33:20Z | - |
| dc.date.issued | 2022-07 | - |
| dc.identifier.uri | http://hdl.handle.net/123456789/1513 | - |
| dc.description.abstract | Imprecise P-element excision or FRT-mediated recombination is routinely performed to mutagenize a gene of interest. It is, however, tedious to maintain all independent and individual excised mutant fly lines before the presence of a mutation is confirmed. Here, we provide a method to detect and confirm the presence of a mutation, as and when mutant flies are generated. By allowing for the maintenance and expansion of only the confirmed mutant lines, this protocol will help to save time, money, and space. | en_US |
| dc.language.iso | en | en_US |
| dc.title | Screening Mutants by Single Fly Genomic PCR | en_US |
| Appears in Collections: | School of Interdisciplinary & Applied Sciences | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 2022-Nagarajan-Georgiou2022_Protocol_ScreeningMutantsMutantsBySingl.pdf | 485.46 kB | Adobe PDF | View/Open |
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